绵羊肺炎支原体延伸因子Tu基因的分子特性分析

doi:10.11843/j.issn.0366-6964.2015.02.016

Abstract

Abstract:

This study aimed at analyzing the molecular characterization of the tuf gene in Mycoplasma ovipneumoniae.The tuf genes of the reference Y98 strain and 11 clinical isolates were cloned and sequenced，and then were used for bioinformatics analysis.A phylogenetic tree was constructed base on the tuf genes.The results showed that the open-reading frames (ORFs) of tuf genes in all the strains were 1 209 bp，which encoded 402 amino acids with 93.3%-100% amino acid identity and possessed higher antigenic index and higher lymphocyte epitopes.The average GC content of the protein coded by tuf gene was 39.80%.The Tuf proteins of reference Y98 strain and 10 clinical isolates contained one strong transmembrane region and were absence of signal peptide，and contained six different functional sites.While another isolate contained two transmembrane regions，with a additional N-glycosylation site in the site of 271-274.The genes from 12 strains were identified 117 single nucleotide polymorphisms (SNPs)，consisting of 41 synonymous polymorphisms and 76 non-synonymous polymorphisms leading to 53 amino acid changes，mainly in 325-354 region near the carboxyl region，which maybe leading to change the function of this protein.The phylogenetic relationship of M.ovipneumoniae based on whole genome and that based on the tuf gene were consistent.So，the tuf gene is a better target than 16S rRNA gene for the phylogenetic analysis for M.ovipneumoniae.Despite of its conservation，the tuf gene still showed genetic diversity within the strains，making it potential for molecular typing.

CLC Number:

S852.62

YIN Zheng-jun，YUE Hua，TANG Cheng. Molecular Characterization of the Elongation Factor Tu Gene in Mycoplasma ovipneumoniae[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 288-294.

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Molecular Characterization of the Elongation Factor Tu Gene in Mycoplasma ovipneumoniae

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